Evaluation of Culture Time and Media in an In Vitro Testis Organ Culture System.

ABSTRACT:

Background

The complexity of spermatogenesis makes development of appropriate in vitro testis models challenging. A novel in vitro mouse testis culture system has been reported but not yet evaluated as an alternative model for male reproductive toxicity testing. We assessed the effects of media composition on sperm differentiation and testis morphology of cultured mouse testis fragments.

Methods

Testes from postnatal day 5 B6:CBA-Tg(Acrv1-EGFP)2727Redd/J male mice were cultured in knockout serum replacement (KSR) or Albumax I (Albumax) medium. Enhanced green fluorescent protein (EGFP) expression was examined on days 35, 42, 45, and 49 of culture. Histology and flow cytometry were performed for testis morphology and spermatid differentiation.

Results

EGFP signals were

SUBMITTER: Nakamura N 

PROVIDER: S-EPMC5462453 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature