A highly conserved sequence of the viral TAP inhibitor ICP47 is required for freezing of the peptide transport cycle.

ABSTRACT: The transporter associated with antigen processing (TAP) translocates antigenic peptides into the endoplasmic reticulum (ER) lumen for loading onto MHC class I molecules. This is a key step in the control of viral infections through CD8+ T-cells. The herpes simplex virus type-1 encodes an 88 amino acid long species-specific TAP inhibitor, ICP47, that functions as a high affinity competitor for the peptide binding site on TAP. It has previously been suggested that the inhibitory function of ICP47 resides within the N-terminal region (residues 1-35). Here we show that mutation of the highly conserved ₅₀PLL₅₂ motif within the central region of ICP47 attenuates its inhibitory capacity. Taking advantage of the human cytomegalovirus-encoded TAP inhibitor US6 as a luminal se

SUBMITTER: Matschulla T 

PROVIDER: S-EPMC5462769 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature