Direct in vivo evidence for increased proliferation of CLL cells in lymph nodes compared to bone marrow and peripheral blood.

ABSTRACT: Chronic lymphocytic leukemia (CLL) is a progressive malignancy of mature B-cells that involves the peripheral blood (PB), lymph nodes (LNs) and bone marrow (BM). Although the majority of CLL cells are in a resting state, small populations of proliferating cells exist; however, the anatomical site of active cell proliferation remains to be definitively determined. Based on findings that CLL cells in LNs have increased expression of B-cell activation genes, we tested the hypothesis that the fraction of 'newly born' cells would be highest in the LNs. Using a deuterium oxide (²H) in vivo labeling method in which patients consumed deuterated (heavy) water (²H₂O), we determined CLL cell kinetics in concurrently obtained samples from LN, PB and BM. The LN was iden

SUBMITTER: Herndon TM 

PROVIDER: S-EPMC5462849 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature