CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.

ABSTRACT: Large genome-mapping consortia and thousands of genome-wide association studies have identified non-protein-coding elements in the genome as having a central role in various biological processes. However, decoding the functions of the millions of putative regulatory elements discovered in these studies remains challenging. CRISPR-Cas9-based epigenome editing technologies have enabled precise perturbation of the activity of specific regulatory elements. Here we describe CRISPR-Cas9-based epigenomic regulatory element screening (CERES) for improved high-throughput screening of regulatory element activity in the native genomic context. Using dCas9^KRAB repressor and dCas9^p300 activator constructs and lentiviral single guide RNA libraries to target DNase I hypersensitive s

SUBMITTER: Klann TS 

PROVIDER: S-EPMC5462860 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature