Long-term Persistence of Innate Lymphoid Cells in the Gut After Intestinal Transplantation.

ABSTRACT:

Background

Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation has not been described.

Methods

Intestinal samples were taken from 4 isolated intestine and 3 multivisceral transplant recipients at the time of any operative resection, such as stoma closure or revision. ILCs were isolated and analyzed by flow cytometry. The target population was defined as being negative for lineage markers and double-positive for CD45/CD127. Cells were further stained to define ILC subsets and a donor-specific or recipient-specific HLA marker to analyze chimerism.

Results

Donor-derived ILCs were found to persist greater than 8 years after transplantation. Additionally, the percentage of ce

SUBMITTER: Weiner J 

PROVIDER: S-EPMC5462871 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature