A Novel Pan-Flavivirus Detection and Identification Assay Based on RT-qPCR and Microarray.

ABSTRACT: The genus Flavivirus includes arthropod-borne viruses responsible for a large number of infections in humans and economically important animals. While RT-PCR protocols for specific detection of most Flavivirus species are available, there has been also a demand for a broad-range Flavivirus assay covering all members of the genus. It is particularly challenging to balance specificity at genus level with equal sensitivity towards each target species. In the present study, a novel assay combining a SYBR Green-based RT-qPCR with a low-density DNA microarray has been developed. Validation experiments confirmed that the RT-qPCR exhibited roughly equal sensitivity of detection and quantification for all flaviviruses tested. These PCR products are subjected to hybridization on

SUBMITTER: Vina-Rodriguez A 

PROVIDER: S-EPMC5463098 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature