Isolation and characterization of the first phage infecting ecologically important marine bacteria Erythrobacter.

ABSTRACT:

Background

Erythrobacter comprises a widespread and ecologically significant genus of marine bacteria. However, no phage infecting Erythrobacter spp. has been reported to date. This study describes the isolation and characterization of phage vB_EliS-R6L from Erythrobacter.

Methods

Standard virus enrichment and double-layer agar methods were used to isolate and characterize the phage. Morphology was observed by transmission electron microscopy, and a one-step growth curve assay was performed. The phage genome was sequenced using the Illumina Miseq platform and annotated using standard bioinformatics tools. Phylogenetic analyses were performed based on the deduced amino acid sequences of terminase, endolysin, portal protein, and major capsid protein, and genome recruitment ana

SUBMITTER: Lu L 

PROVIDER: S-EPMC5463345 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature