Gene-centromere mapping in meiotic gynogenetic European seabass.

ABSTRACT:

Background

Fully isogenic lines in fish can be developed using "mitotic" gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families.

Results

From a single meiotic gynogenetic family consisting of 79 progeny, 42 million sequencing r

SUBMITTER: Oral M 

PROVIDER: S-EPMC5463376 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature