Performances of Different Fragment Sizes for Reduced Representation Bisulfite Sequencing in Pigs.

ABSTRACT:

Background

Reduced representation bisulfite sequencing (RRBS) has been widely used to profile genome-scale DNA methylation in mammalian genomes. However, the applications and technical performances of RRBS with different fragment sizes have not been systematically reported in pigs, which serve as one of the important biomedical models for humans. The aims of this study were to evaluate capacities of RRBS libraries with different fragment sizes to characterize the porcine genome.

Results

We found that the MspI-digested segments between 40 and 220 bp harbored a high distribution peak at 74 bp, which were highly overlapped with the repetitive elements and might reduce the unique mapping alignment. The RRBS library of 110-220 bp fragment size had the highest unique mappin

SUBMITTER: Yuan XL 

PROVIDER: S-EPMC5463379 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature