IL-6 Inhibits Upregulation of Membrane-Bound TGF-β 1 on CD4+ T Cells and Blocking IL-6 Enhances Oral Tolerance.

ABSTRACT: Oral administration of Ag induces regulatory T cells that express latent membrane-bound TGF-β (latency-associated peptide [LAP]) and have been shown to play an important role in the induction of oral tolerance. We developed an in vitro model to study modulation of LAP⁺ on CD4⁺ T cells. The combination of anti-CD3 mAb, anti-CD28 mAb, and recombinant IL-2 induced expression of LAP on naive CD4⁺ T cells, independent of Foxp3 or exogenous TGF-β. In vitro generated CD4⁺LAP⁺Foxp3^- T cells were suppressive in vitro, inhibiting proliferation of naive CD4⁺ T cells and IL-17A secretion by Th17 cells. Assessing the impact of different cytokines and neutralizing Abs against cytokines, we found that LAP induction was decreased

SUBMITTER: Kuhn C 

PROVIDER: S-EPMC5463579 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature