A novel 3D human glioblastoma cell culture system for modeling drug and radiation responses.

ABSTRACT:

Background

Glioblastoma (GBM) is the most common primary brain tumor, with dismal prognosis. The failure of drug-radiation combinations with promising preclinical data to translate into effective clinical treatments may relate to the use of simplified 2-dimensional in vitro GBM cultures.

Methods

We developed a customized 3D GBM culture system based on a polystyrene scaffold (Alvetex) that recapitulates key histological features of GBM and compared it with conventional 2D cultures with respect to their response to radiation and to molecular targeted agents for which clinical data are available.

Results

In 3 patient-derived GBM lines, no difference in radiation sensitivity was observed between 2D and 3D cultures, as measured by clonogenic survival. Three different molec

SUBMITTER: Gomez-Roman N 

PROVIDER: S-EPMC5463789 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature