A tool named Iris for versatile high-throughput phenotyping in microorganisms.

ABSTRACT: Advances in our ability to systematically introduce and track controlled genetic variance in microorganisms have, in the past decade, fuelled high-throughput reverse genetics approaches. When coupled to quantitative readouts, such approaches are extremely powerful at elucidating gene function and providing insights into the underlying pathways and the overall cellular network organization. Yet, until now, all efforts to quantify microbial macroscopic phenotypes have been restricted to monitoring growth in a small number of model microorganisms. We have developed an image analysis software named Iris, which allows for systematic exploration of a number of orthogonal-to-growth processes, including biofilm formation, colony morphogenesis, envelope biogenesis, sporulation and reporter activity

SUBMITTER: Kritikos G 

PROVIDER: S-EPMC5464397 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature