Dissecting inherent intratumor heterogeneity in patient-derived glioblastoma culture models.

ABSTRACT:

Background

Molecular profile of glioblastoma multiforme (GBM) revealed 4 subtypes, 2 of which, proneural and mesenchymal, have been predominantly observed, with the latter displaying a more aggressive phenotype and increased therapeutic resistance. Single-cell RNA sequencing revealed that multiple subtypes actually reside within the same tumor, suggesting cellular heterogeneity in GBM. Further, plasticity between these 2 subtypes is observed during tumor recurrence and in response to radiation therapy.

Methods

Patient-derived GBM stemlike cells were cultured as neurospheres. These cells were differentiated in serum by attaching to the culture dishes. The "floating" cells that were not attached/differentiated were harvested from the conditioned medium. The characteristics of

SUBMITTER: Teng J 

PROVIDER: S-EPMC5464448 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature