Polymorphisms in the yeast galactose sensor underlie a natural continuum of nutrient-decision phenotypes.

ABSTRACT: In nature, microbes often need to "decide" which of several available nutrients to utilize, a choice that depends on a cell's inherent preference and external nutrient levels. While natural environments can have mixtures of different nutrients, phenotypic variation in microbes' decisions of which nutrient to utilize is poorly studied. Here, we quantified differences in the concentration of glucose and galactose required to induce galactose-responsive (GAL) genes across 36 wild S. cerevisiae strains. Using bulk segregant analysis, we found that a locus containing the galactose sensor GAL3 was associated with differences in GAL signaling in eight different crosses. Using allele replacements, we confirmed that GAL3 is the major driver of GAL induction variation, and that GAL3 allelic variatio

SUBMITTER: Lee KB 

PROVIDER: S-EPMC5464677 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature