Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system.

ABSTRACT: Legionnaires' disease, predominantly caused by the bacterium Legionella pneumophila, has increased in prevalence worldwide. The most common mode of transmission of Legionella is inhalation of contaminated aerosols, such as those generated by cooling towers. Simple, rapid and accurate methods to enumerate L. pneumophila are required to prevent the spread of this organism. Here, we applied a microfluidic device for on-chip fluorescent staining and semi-automated counting of L. pneumophila in cooling tower water. We also constructed a portable system for rapid on-site monitoring and used it to enumerate target bacterial cells rapidly flowing in the microchannel. A fluorescently-labelled polyclonal antibody was used for the selective detection of L. pneumophila serogroup 1 in the samples. The

SUBMITTER: Yamaguchi N 

PROVIDER: S-EPMC5465085 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature