Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium.

ABSTRACT: Members of the yeast family of PUF proteins bind unique subsets of mRNA targets that encode proteins with common functions. They therefore became a paradigm for post-transcriptional gene control. To provide new insights into the roles of the seemingly redundant Puf1 and Puf2 members, we monitored the growth rates of their deletions under many different stress conditions. A differential effect was observed at high CaCl₂ concentrations, whereby puf1Δ growth was affected much more than puf2Δ, and inhibition was exacerbated in puf1Δpuf2Δ double knockout. Transcriptome analyses upon CaCl₂ application for short and long terms defined the transcriptional response to CaCl₂ and revealed distinct expression changes for the deletions. Intriguingly, mRNAs known to be b

SUBMITTER: Haramati O 

PROVIDER: S-EPMC5465220 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature