Development of an objective gene expression panel as an alternative to self-reported symptom scores in human influenza challenge trials.

ABSTRACT:

Background

Influenza challenge trials are important for vaccine efficacy testing. Currently, disease severity is determined by self-reported scores to a list of symptoms which can be highly subjective. A more objective measure would allow for improved data analysis.

Methods

Twenty-one volunteers participated in an influenza challenge trial. We calculated the daily sum of scores (DSS) for a list of 16 influenza symptoms. Whole blood collected at baseline and 24, 48, 72 and 96 h post challenge was profiled on Illumina HT12v4 microarrays. Changes in gene expression most strongly correlated with DSS were selected to train a Random Forest model and tested on two independent test sets consisting of 41 individuals profiled on a different microarray platform and 33 volunteers assaye

SUBMITTER: Muller J 

PROVIDER: S-EPMC5465537 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature