Erythrocyte sialoglycoproteins engage Siglec-9 on neutrophils to suppress activation.

ABSTRACT: Healthy blood neutrophils are functionally quiescent in the bloodstream, have a short lifespan, and exit the circulation to carry out innate immune functions, or undergo rapid apoptosis and macrophage-mediated clearance to mitigate host tissue damage. Limitation of unnecessary intravascular neutrophil activation is also important to prevent serious inflammatory pathologies. Because neutrophils become easily activated after purification, we carried out ex vivo comparisons with neutrophils maintained in whole blood. We found a difference in activation state, with purified neutrophils showing signs of increased reactivity: shedding of l-selectin, CD11b upregulation, increased oxidative burst, and faster progression to apoptosis. We discovered that erythrocytes suppressed neutrophil activation

SUBMITTER: Lizcano A 

PROVIDER: S-EPMC5465837 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature