Pulses of Ca²⁺ coordinate actin assembly and exocytosis for stepwise cell extension.

ABSTRACT: Many eukaryotic cells grow by extending their cell periphery in pulses. The molecular mechanisms underlying this process are not yet fully understood. Here we present a comprehensive model of stepwise cell extension by using the unique tip growth system of filamentous fungi. Live-cell imaging analysis, including superresolution microscopy, revealed that the fungus Aspergillus nidulans extends the hyphal tip in an oscillatory manner. The amount of F-actin and secretory vesicles (SV) accumulating at the hyphal tip oscillated with a positive temporal correlation, whereas vesicle amounts were negatively correlated to the growth rate. The intracellular Ca²⁺ level also pulsed with a positive temporal correlation to the amount of F-actin and SV at the hyphal tip. Two Ca^2+</

SUBMITTER: Takeshita N 

PROVIDER: S-EPMC5465874 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature