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Increasing intracellular magnesium levels with the 31-amino acid MgtS protein.


ABSTRACT: Synthesis of the 31-amino acid, inner membrane protein MgtS (formerly denoted YneM) is induced by very low Mg2+ in a PhoPQ-dependent manner in Escherichia coli Here we report that MgtS acts to increase intracellular Mg2+ levels and maintain cell integrity upon Mg2+ depletion. Upon development of a functional tagged derivative of MgtS, we found that MgtS interacts with MgtA to increase the levels of this P-type ATPase Mg2+ transporter under Mg2+-limiting conditions. Correspondingly, the effects of MgtS upon Mg2+ limitation are lost in a ∆mgtA mutant, and MgtA overexpression can suppress the ∆mgtS phenotype. MgtS stabilization of MgtA provides an additional layer of regulation of this tightly controlled Mg2+ transporter and adds to the list of small proteins that regulate inner membrane transporters.

SUBMITTER: Wang H 

PROVIDER: S-EPMC5465934 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Increasing intracellular magnesium levels with the 31-amino acid MgtS protein.

Wang Hanbo H   Yin Xuefeng X   Wu Orr Mona M   Dambach Michael M   Curtis Rebecca R   Storz Gisela G  

Proceedings of the National Academy of Sciences of the United States of America 20170516 22


Synthesis of the 31-amino acid, inner membrane protein MgtS (formerly denoted YneM) is induced by very low Mg<sup>2+</sup> in a PhoPQ-dependent manner in <i>Escherichia coli</i> Here we report that MgtS acts to increase intracellular Mg<sup>2+</sup> levels and maintain cell integrity upon Mg<sup>2+</sup> depletion. Upon development of a functional tagged derivative of MgtS, we found that MgtS interacts with MgtA to increase the levels of this P-type ATPase Mg<sup>2+</sup> transporter under Mg<su  ...[more]

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