A new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples.

ABSTRACT: A microfluidic method to specifically capture and detect infectious bacteria based on immunorecognition and proliferative power is presented. It involves a microscale fluidized bed in which magnetic and drag forces are balanced to retain antibody-functionalized superparamagnetic beads in a chamber during sample perfusion. Captured cells are then cultivated in situ by infusing nutritionally-rich medium. The system was validated by the direct one-step detection of Salmonella Typhimurium in undiluted unskimmed milk, without pre-treatment. The growth of bacteria induces an expansion of the fluidized bed, mainly due to the volume occupied by the newly formed bacteria. This expansion can be observed with the naked eye, providing simple low-cost detection of only a few bacteria and

SUBMITTER: Pereiro I 

PROVIDER: S-EPMC5465951 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature