Umbilical Cord Blood NOS1 as a Potential Biomarker of Neonatal Encephalopathy.

ABSTRACT:

Background

There are no definitive markers to aid in diagnosis of neonatal encephalopathy (NE). The purpose of our study was (1) to identify and evaluate the utility of neuronal nitric oxide synthase (NOS1) in umbilical cord blood as a NE biomarker and (2) to identify the source of NOS1 in umbilical cord blood.

Methods

This was a nested case-control study of neonates >35 weeks of gestation. ELISA for NOS1 in umbilical cord blood was performed. Sources of NOS1 in umbilical cord were investigated by immunohistochemistry, western blot, ELISA, and quantitative PCR. Furthermore, umbilical cords of full-term neonates were subjected to 1% hypoxia ex vivo.

Results

NOS1 was present in umbilical cord blood and increased in NE cases compared with controls. NOS1 was expres

SUBMITTER: Lei J 

PROVIDER: S-EPMC5466059 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature