Laccase production by Pleurotus ostreatus and its application in synthesis of gold nanoparticles.

ABSTRACT: In this work, the production of fungal laccase was optimized from local isolate of Pleurotus ostreatus using solid state fermentation. Factorial design was used to study the effect of several nutrients on enzyme production. Purification and characterization of the enzyme and the effect of temperature, pH and gamma radiation on fungal growth and enzyme production was investigated. Optimization of production conditions yielded an enzyme with activity over 32,450 IU/g of fermented substrate. Factorial design was capable of establishing the conditions that multiplied the activity of the enzyme several folds, consequently, reducing the cost of production. The enzyme was capable of decolorizing several dyes with over 80% reduction in color confirming the aromatic degrading capabili

SUBMITTER: El-Batal AI 

PROVIDER: S-EPMC5466191 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature