Relative efficiency of zinc sulfide (ZnS) quantum dots (QDs) based electrochemical and fluorescence immunoassay for the detection of Staphylococcal enterotoxin B (SEB).

ABSTRACT: In this paper an attempt was made to detect Staphylococcal enterotoxin B (SEB) both by electrochemical and fluorescence immunoassay methods using zinc sulphide (ZnS) QDs. Wet-chemical method was adopted for the preparation of fluorescent ZnS QDs (diameter ∼ 5-10 nm). These QDs were bioconjugated with monoclonal antibodies and then characterized by various method. A detection limit of 0.02 ng mL^-1 by fluorescence assay and 1.0 ng mL^-1 by electrochemical assay for SEB was achieved. While by sandwich ELISA it is possible to detect 0.24 ng mL^-1 only. The sensitivity of all techniques is very good, since the LD₅₀ of SEB is 20 ng kg^-1. Electrochemical assay is faster, need low-cost instrument, independent to the size of QDs and found

SUBMITTER: Sharma A 

PROVIDER: S-EPMC5466261 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature