Extracellular expression of alkaline phytase in Pichia pastoris: Influence of signal peptides, promoters and growth medium.

ABSTRACT: Alkaline phytase isolated from pollen grains of Lilium longiflorum (LlALP) possesses unique catalytic and thermal stability properties that suggest it has the potential to be used as a feed supplement. However, substantial amounts of active enzymes are needed for animal feed studies and endogenous levels of LlALP in lily pollen are too low to provide the required amounts. Active rLlALP2 (coded by LlAlp2, one of two isoforms of alkaline phytase cDNA identified in lily pollen) has been successfully expressed in intracellular compartments of Pichia pastoris, however enzyme yields have been modest (25-30 mg/L) and purification of the enzyme has been challenging. Expression of foreign proteins to the extracellular medium of P. pastoris greatly simplifies protein puri

SUBMITTER: Yang M 

PROVIDER: S-EPMC5466264 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature