Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker.

ABSTRACT: Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, D_LOB 2, D_LOB 3, D_LOB 9, D_LOB 10, D

SUBMITTER: Jia H 

PROVIDER: S-EPMC5466436 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature