Regulation of gene expression by manipulating transcriptional repressor activity using a novel CoSRI technology.

ABSTRACT: Targeted gene manipulation is a central strategy for studying gene function and identifying related biological processes. However, a methodology for manipulating the regulatory motifs of transcription factors is lacking as these factors commonly possess multiple motifs (e.g. repression and activation motifs) which collaborate with each other to regulate multiple biological processes. We describe a novel approach designated conserved sequence-guided repressor inhibition (CoSRI) that can specifically reduce or abolish the repressive activities of transcription factors in vivo. The technology was evaluated using the chimeric MYB80-EAR transcription factor and subsequently the endogenous WUS transcription factor. The technology was employed to develop a reversible male sterility system applica

SUBMITTER: Xu Y 

PROVIDER: S-EPMC5466438 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature