DrVM: a new tool for efficient genome assembly of known eukaryotic viruses from metagenomes.

ABSTRACT:

Background

Virus discovery using high-throughput next-generation sequencing has become more commonplace. However, although analysis of deep next-generation sequencing data allows us to identity potential pathogens, the entire analytical procedure requires competency in the bioinformatics domain, which includes implementing proper software packages and preparing prerequisite databases. Simple and user-friendly bioinformatics pipelines are urgently required to obtain complete viral genome sequences from metagenomic data.

Results

This manuscript presents a pipeline, drVM (detect and reconstruct known viral genomes from metagenomes), for rapid viral read identification, genus-level read partition, read normalization, de novo assembly, sequence annotation, and coverage profiling.

SUBMITTER: Lin HH 

PROVIDER: S-EPMC5466706 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature