Comparing intra-articular CTXII levels assessed via magnetic capture or lavage in a rat knee osteoarthritis model.

ABSTRACT:

Objective

Parallel measures of osteoarthritis (OA) across species can help evaluate OA models relative to humans. Toward this need, our group recently developed a magnetic nanoparticle-based technology, termed magnetic capture, to analyze biomarkers within a rat knee. The objectives of this study were to directly compare magnetic capture to lavage, and assess c-telopeptide of collagen type II (CTXII) in the rat medial meniscus transection (MMT) model of knee OA.

Design

MMT surgery was performed in 30 male Lewis rats (3 months, 250 g). Using lavage or magnetic capture, CTXII was assessed in the OA-affected and contralateral knee at 1 week (n = 6 per group) or 4 weeks (n = 8 per group) after surgery.

Results

While lavage detected elevated CTXII concentrations in the OA-

SUBMITTER: Yarmola EG 

PROVIDER: S-EPMC5466845 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature