A reference gene set construction using RNA-seq of multiple tissues of Chinese giant salamander, Andrias davidianus.

ABSTRACT:

Background

Chinese giant salamander (CGS) is the largest extant amphibian species in the world. Owing to its evolutionary position and four peculiar phenomenon of life (longevity, starvation tolerance, regenerative ability, and hatch without sunshine), it is an invaluable model species for research. However, lack of genomic resources leads to fewer study progresses in these fields, due to its huge genome of ∼50 GB making it extremely difficult to be assembled.

Results

We reported the sequenced transcriptome of more than 20 tissues from adult CGS using Illumina Hiseq 2000 technology, and a total of 93 366 no-redundancy transcripts with a mean length of 1326 bp were obtained. We developed for the first time an efficient pipeline to construct a high-quality reference gene set o

SUBMITTER: Geng X 

PROVIDER: S-EPMC5467019 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature