The use of Oxford Nanopore native barcoding for complete genome assembly.

ABSTRACT:

Background

The Oxford Nanopore Technologies MinION(TM) is a mobile DNA sequencer that can produce long read sequences with a short turn-around time. Here we report the first demonstration of single contig genome assembly using Oxford Nanopore native barcoding when applied to a multiplexed library of 12 samples and combined with existing Illumina short read data. This paves the way for the closure of multiple bacterial genomes from a single MinION(TM) sequencing run, given the availability of existing short read data. The strain we used, MHO_001, represents the important community-acquired methicillin-resistant Staphylococcus aureus lineage USA300.

Findings

Using a hybrid assembly of existing short read and barcoded long read sequences from multiplexed data, we completed a ge

SUBMITTER: Bayliss SC 

PROVIDER: S-EPMC5467021 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature