Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes.

ABSTRACT: Understanding the function of the thousands of cellular proteins is a central question in molecular cell biology. As proteins are typically part of multiple dynamic and often overlapping macromolecular complexes exerting distinct functions, the identification of protein-protein interactions (PPI) and their assignment to specific complexes is a crucial but challenging task. We present a protein fragments complementation assay integrated with the proximity-dependent biotinylation technique BioID. Activated on the interaction of two proteins, split-BioID is a conditional proteomics approach that allows in a single and simple assay to both experimentally validate binary PPI and to unbiasedly identify additional interacting factors. Applying our method to the miRNA-mediated silencing pathway, w

SUBMITTER: Schopp IM 

PROVIDER: S-EPMC5467174 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature