Conformational Transitions and Alternating-Access Mechanism in the Sarcoplasmic Reticulum Calcium Pump.

ABSTRACT: Ion pumps are integral membrane proteins responsible for transporting ions against concentration gradients across biological membranes. Sarco/endoplasmic reticulum Ca²⁺-ATPase (SERCA), a member of the P-type ATPases family, transports two calcium ions per hydrolyzed ATP molecule via an "alternating-access" mechanism. High-resolution crystallographic structures provide invaluable insight on the structural mechanism of the ion pumping process. However, to understand the molecular details of how ATP hydrolysis is coupled to calcium transport, it is necessary to gain knowledge about the conformational transition pathways connecting the crystallographically resolved conformations. Large-scale transitions in SERCA occur at time-scales beyond the current reach of unbiased molecular dyn

SUBMITTER: Das A 

PROVIDER: S-EPMC5467534 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature