Kv7 Channel Activation Underpins EPAC-Dependent Relaxations of Rat Arteries.

ABSTRACT:

Objective

To establish the role of Kv7 channels in EPAC (exchange protein directly activated by cAMP)-dependent relaxations of the rat vasculature and to investigate whether this contributes to β-adrenoceptor-mediated vasorelaxations.

Approach and results

Isolated rat renal and mesenteric arteries (RA and MA, respectively) were used for isometric tension recording to study the relaxant effects of a specific EPAC activator and the β-adrenoceptor agonist isoproterenol in the presence of potassium channel inhibitors and cell signaling modulators. Isolated myocytes were used in proximity ligation assay studies to detect localization of signaling intermediaries with Kv7.4 before and after cell stimulation. Our studies showed that the EPAC activator (8-pCPT-2Me-cAMP-AM) produced r

SUBMITTER: Stott JB 

PROVIDER: S-EPMC5467728 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature