Classical complement pathway activation in the nasal tissue of patients with chronic rhinosinusitis.

ABSTRACT:

Background

Complement plays a major role in inflammatory diseases, but its involvement and mechanisms of activation in patients with chronic rhinosinusitis (CRS) are not known.

Objectives

After earlier studies discovering autoantibodies in patients with CRS, we sought to investigate the nature, extent, and location of complement activation in nasal tissue of patients with CRS. Specifically, we were interested in whether antibody-mediated activation through the classical pathway was a major mechanism for complement activation in patients with CRS.

Methods

Nasal tissue was obtained from patients with CRS and control subjects. Tissue homogenates were analyzed for complement activation products (ELISA-C5b-9, C4d, activated C1, and C5a) and major complement-fixing antibodi

SUBMITTER: Van Roey GA 

PROVIDER: S-EPMC5468513 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature