A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination.

ABSTRACT:

Background

Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens that causes severe economic losses in the poultry industry worldwide. Major advances in the study of the molecular biology of IBV have resulted from the development of reverse genetics systems for the highly attenuated, cell culture-adapted, IBV strain Beaudette. However, most IBV strains, amongst them virulent field isolates, can only be propagated in embryonated chicken eggs, and not in continuous cell lines.

Methods

We established a reverse genetics system for the IBV strain H52, based on targeted RNA recombination in a two-step process. First, a genomic and a chimeric synthetic, modified IBV RNA were co-transfected into non-susceptible cells to generate a recombinant chim

SUBMITTER: van Beurden SJ 

PROVIDER: S-EPMC5468965 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature