Development and pharmacologic characterization of the rat 6 Hz model of partial seizures.

ABSTRACT:

Objective

The mouse 6 Hz model of psychomotor seizures is a well-established and commonly used preclinical model for antiseizure drug (ASD) discovery. Despite its widespread use both in the identification and differentiation of novel ASDs in mice, a corresponding assay in rats has not been developed. We established a method for 6 Hz seizure induction in rats, with seizure behaviors similar to those observed in mice including head nod, jaw clonus, and forelimb clonus.

Methods

A convulsive current that elicits these seizure behaviors in 97% of rats (CC₉₇ ) was determined using a Probit analysis. Numerous prototype ASDs were evaluated in this model using stimulus intensities of 1.5× and 2× the CC₉₇ , which is comparable to the approach used in the mouse 6

SUBMITTER: Metcalf CS 

PROVIDER: S-EPMC5469205 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature