High Sensitivity Determination of TNF-α for Early Diagnosis of Neonatal Infections with a Novel and Reusable Electrochemical Sensor.

ABSTRACT: Early diagnosis is vital for the reduction of mortality caused by neonatal infections. Since TNF-α can be used as a marker for the early diagnosis, the detection of TNF-α with high sensitivity and specificity has great clinical significance. Herein, a highly sensitive and reusable electrochemical sensor was fabricated. Due to the high specificity of aptamers, TNF-α could be accurately detected from five similar cytokines, even from serum samples. In addition, Au nanoparticles (AuNPs) with a high surface area were able to combine a large number of doxorubicin hydrochloride (DOXh), which made the sensor have a high sensitivity. The sensor had a good linear relationship with TNF-α concentration in the range from 1 to 1 × 10⁴ pg/mL and the lowest detection limit is 0.7 pg/mL. More important wa

SUBMITTER: Li L 

PROVIDER: S-EPMC5469345 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature