Serum microRNAs are altered in various stages of chronic kidney disease: a preliminary study.

ABSTRACT:

Background

MicroRNAs (miRNAs) are innovative and informative blood-based biomarkers involved in numerous pathophysiological processes. In this study and based on our previous experimental data, we investigated miR-126, miR-143, miR-145, miR-155 and miR-223 as potential circulating biomarkers for the diagnosis and prognosis of patients with chronic kidney disease (CKD). The primary objective of this study was to assess the levels of miRNA expression at various stages of CKD.

Methods

RNA was extracted from serum, and RT-qPCR was performed for the five miRNAs and cel-miR-39 (internal control).

Results

Serum levels of miR-143, -145 and -223 were elevated in patients with CKD compared with healthy controls. They were further increased in chronic haemodialysis patien

SUBMITTER: Brigant B 

PROVIDER: S-EPMC5469576 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature