Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.

ABSTRACT: The regeneration blastema which forms following amputation of the mouse digit tip is composed of undifferentiated cells bound together by an organized network of fibers. A monoclonal antibody (ER-TR7) that identifies extracellular matrix (ECM) fibers produced by fibroblast reticular cells during lymphoid organogenesis was used to characterize the ECM of the digit, the blastema, and the regenerate. Digit fibroblast reticular cells produce an ER-TR7⁺ ECM network associated with different tissues and represent a subset of loose connective tissue fibroblasts. During blastema formation there is an upregulation of matrix production that returns to its pre-existing level and anatomical pattern in the endpoint regenerate. Co-localization studies demonstrate a strong spatial correlation

SUBMITTER: Marrero L 

PROVIDER: S-EPMC5469731 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature