Soil biochar amendment affects the diversity of nosZ transcripts: Implications for N₂O formation.

ABSTRACT: Microbial nitrogen transformation processes such as denitrification represent major sources of the potent greenhouse gas nitrous oxide (N₂O). Soil biochar amendment has been shown to significantly decrease N₂O emissions in various soils. However, the effect of biochar on the structure and function of microbial communities that actively perform nitrogen redox transformations has not been studied in detail yet. To analyse the community composition of actively denitrifying and N₂O-reducing microbial communities, we collected RNA samples at different time points from a soil microcosm experiment conducted under denitrifying conditions and performed Illumina amplicon sequencing targeting nirK, typical nosZ and atypical nosZ mRNA transcripts. Within 10 days, bioch

SUBMITTER: Harter J 

PROVIDER: S-EPMC5469825 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature