SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells.

ABSTRACT: Blood cell generation depends on continuous cellular output by the sequential hierarchy of hematopoietic stem cell (HSC) and progenitor populations that all contain quiescent and actively cycling cells. Hematopoietic stem and progenitor cells (HSPCs) express the surface molecule Stem cell antigen 1 (SCA-1/LY6A). Using histone 2B-red fluorescent fusion protein label retention and cell-cycle reporter mice, we demonstrate that high SCA-1 expression (SCA-1^hi) identifies not only quiescent HSCs but quiescent cells on all hierarchical levels within the lineage^-SCA-1⁺KIT⁺ (LSK) population. Each transplanted SCA-1^hi HSPC population also displayed self-renewal potential superior to that of the respective SCA-1^lo population. SCA-1 exp

SUBMITTER: Morcos MNF 

PROVIDER: S-EPMC5469944 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature