Cxcl8b and Cxcr2 Regulate Neutrophil Migration through Bloodstream in Zebrafish.

ABSTRACT: Neutrophils play an essential role during an inflammatory response, which is dependent on their rapid recruitment from the bone marrow to the vasculature. However, there is no information about the molecular signals that regulate neutrophil entry to circulation during an inflammatory process in humans. This is mainly due to the lack of a suitable model of study that contains similar set of molecules and that allows in vivo analyses. In this study, we used the zebrafish to assess the role of Cxcl8a, Cxcl8b, and Cxcr2 in neutrophil migration to blood circulation after injury. Using Tg(BACmpx:GFP)ⁱ¹¹⁴ transgenic embryos and two damage models (severe and mild), we developed in vivo lack of function assays. We found that the transcription levels of cxcl8a, cxcl8b, and <

SUBMITTER: Zuniga-Traslavina C 

PROVIDER: S-EPMC5470028 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature