Biological Effects of Phosphocitrate on Osteoarthritic Articular Chondrocytes.

ABSTRACT:

Background

Phosphocitrate (PC) inhibits osteoarthritis (OA) in Hartley guinea pigs. However, the underlying molecular mechanisms remain poorly understood.

Objective

This study sought to examine the biological effect of PC on OA chondrocytes and test the hypothesis that PC may exert its OA disease modifying effect, in part, by inhibiting the expression of genes implicated in OA disease process and stimulating the production of extracellular matrices.

Method

OA chondrocytes were cultured in the absence or presence of PC. Total RNA was extracted and subjected to microarray analyses. The effect of PC on proliferation and chondrocyte-mediated calcification were examined in monolayer culture. The effect of PC on the production of extracellular matrices was examined in micro

SUBMITTER: Sun Y 

PROVIDER: S-EPMC5470061 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature