Ontology highlight
ABSTRACT:
SUBMITTER: Mou H
PROVIDER: S-EPMC5470253 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Genome biology 20170614 1
CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of β-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larg ...[more]