Impact of protein kinase CK2 inhibitors on proliferation and differentiation of neural stem cells.

ABSTRACT:

Background

Protein kinases play central roles in cell and tissue development. Protein kinase CK2, an ubiquitously expressed serine/threonine kinase has severe impacts on embryo- and spermatogenesis. Since its role in neurogenesis has so far only been investigated in very few studies, we analysed the role of CK2 in neural stem cells by using two specific inhibitors.

Methods

Neural stem cells were isolated from the subventricular zone of neonatal mice, using a neurosphere approach. Proliferation of the neurospheres, as well as their differentiation was investigated with and without inhibition of CK2. Changes in proliferation were assessed by counting the number and measuring the diameter of the neurospheres. Furthermore, the absolute cell numbers within the neurospheres were e

SUBMITTER: Bender M 

PROVIDER: S-EPMC5470557 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature