Comparative analysis of virus-host interactions caused by a virulent and an attenuated duck hepatitis A virus genotype 1.

ABSTRACT: Because of their better immunogenicity and the improved protection they afford, live attenuated vaccines derived from serial passaging in an abnormal host are widely used to protect humans or animals from certain pathogens. Here, we used a virulent and a chicken embryo-attenuated duck hepatitis A virus genotype 1 to compare the different regulated immune responses induced by viruses with differing virulence. In this study, the attenuated strains had lower protein expression levels than the virulent strains as identified by immunohistochemistry. This may be caused by apparent codon usage bias selected during passage. Furthermore, lower translation efficiency led to decreased viral replication, which is highly dependent on non-structural viral protein expression. Although the two strains had

SUBMITTER: Ou X 

PROVIDER: S-EPMC5470708 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature