Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators.

ABSTRACT: This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using 'U' shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded in the HNR preventing direct in-situ electrophoretic separation. The flow of analytes through the HNR was achieved by balancing the hydrodynamic pressure to override the electromotive force and inhibit the motion of analytes towards the anode for capillary electrophoresis. The resonance frequency shifts of the HNR vibrating around 1.5 MHz were correlated with the time of the passage of the protein bands to construct the mass spectrum. The proposed concept was demonstrated by constructing a mass spectrum o

SUBMITTER: Chaudhari S 

PROVIDER: S-EPMC5471201 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature