Serotyping dengue virus with isothermal amplification and a portable sequencer.

ABSTRACT: The recent development of a nanopore-type portable DNA sequencer has changed the way we think about DNA sequencing. We can perform sequencing directly in the field, where we collect the samples. Here, we report the development of a novel method to detect and genotype tropical disease pathogens, using dengue fever as a model. By combining the sequencer with isothermal amplification that only requires a water bath, we were able to amplify and sequence target viral genomes with ease. Starting from a serum sample, the entire procedure could be finished in a single day. The analysis of blood samples collected from 141 Indonesian patients demonstrated that this method enables the clinical identification and serotyping of the dengue virus with high sensitivity and specificity. The overall success

SUBMITTER: Yamagishi J 

PROVIDER: S-EPMC5471244 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature