Integrative approach for the analysis of the proteome-wide response to bismuth drugs in Helicobacter pylori.

ABSTRACT: Bismuth drugs, despite being clinically used for decades, surprisingly remain in use and effective for the treatment of Helicobacter pylori infection, even for resistant strains when co-administrated with antibiotics. However, the molecular mechanisms underlying the clinically sustained susceptibility of H. pylori to bismuth drugs remain elusive. Herein, we report that integration of in-house metalloproteomics and quantitative proteomics allows comprehensive uncovering of the bismuth-associated proteomes, including 63 bismuth-binding and 119 bismuth-regulated proteins from Helicobacter pylori, with over 60% being annotated with catalytic functions. Through bioinformatics analysis in combination with bioassays, we demonstrated that bismuth drugs disrupted multiple essen

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5471454 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature